DOI: 10.1073/pnas.91.24.11728. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin.

Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin.

10.1073/pnas.91.24.11728

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Caveolae are small invaginations of the plasma membrane 50-100 nm in diameter. Since calcium channels, inositol 1,4,5-trisphosphate receptors, and heterotrimeric GTP-binding proteins (G proteins) are localized in caveolae, they may participate in signal transduction by G protein-coupled receptors. Here we show that the G protein-coupled endothelin receptor subtype A (ETA) and its bound endothelin ligand are found in plasma membrane caveolae. ETA and its bound ligand coimmunoprecipitate with caveolin, a structural component of caveolae, in extracts of cells expressing transfected ETA receptors. Confocal fluorescence microscopy shows colocalization of ETA receptors and caveolin in micropatches at or near the plasma membrane, in the absence of endothelin ligands. These observations demonstrate a functional role for plasma membrane caveolae in signal transduction by this G protein-coupled receptor.

Bibliography

Chun, M., Liyanage, U. K., Lisanti, M. P., & Lodish, H. F. (1994). Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proceedings of the National Academy of Sciences, 91(24), 11728â11732.

Authors 4

M Chun (first)
U K Liyanage (additional)
M P Lisanti (additional)
H F Lodish (additional)

References 0 Referenced 246

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:59 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:58 p.m.)
Indexed	3 months ago (May 20, 2025, 9:31 a.m.)
Issued	30 years, 9 months ago (Nov. 22, 1994)
Published	30 years, 9 months ago (Nov. 22, 1994)
Published Online	30 years, 9 months ago (Nov. 22, 1994)
Published Print	30 years, 9 months ago (Nov. 22, 1994)

Funders 0

None

BibTeX

@article{Chun_1994, title={Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.24.11728}, DOI={10.1073/pnas.91.24.11728}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chun, M and Liyanage, U K and Lisanti, M P and Lodish, H F}, year={1994}, month=nov, pages={11728–11732} }

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