DOI: 10.1073/pnas.91.17.8170. Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel.

Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel.

10.1073/pnas.91.17.8170

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices. During focal synaptic stimulation, one or more highly localized regions of Ca2+ elevation (hot spots) were detected in the dendrites. Ca2+ spread from the center of hot spots with properties consistent with diffusion. Several lines of evidence indicate that these hot spots were due to Ca2+ entry through N-methyl-D-aspartate synaptic channels. The spatial and temporal resolution of the method was sufficient to detect the response of single hot spots to single stimuli, thus providing a real-time method for monitoring local synaptic activity. Using this method, we show that synapses on the same dendrite differ in their probability of response and in their facilitation properties.

Bibliography

Malinow, R., Otmakhov, N., Blum, K. I., & Lisman, J. (1994). Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel. Proceedings of the National Academy of Sciences, 91(17), 8170â8174.

Authors 4

R Malinow (first)
N Otmakhov (additional)
K I Blum (additional)
J Lisman (additional)

References 0 Referenced 64

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:52 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:50 p.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:43 a.m.)
Issued	31 years ago (Aug. 16, 1994)
Published	31 years ago (Aug. 16, 1994)
Published Online	31 years ago (Aug. 16, 1994)
Published Print	31 years ago (Aug. 16, 1994)

Funders 0

None

BibTeX

@article{Malinow_1994, title={Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.17.8170}, DOI={10.1073/pnas.91.17.8170}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Malinow, R and Otmakhov, N and Blum, K I and Lisman, J}, year={1994}, month=aug, pages={8170–8174} }

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