DOI: 10.1073/pnas.91.15.6845. Rearrangement of the histone H2A C-terminal domain in the nucleosome.

Rearrangement of the histone H2A C-terminal domain in the nucleosome.

10.1073/pnas.91.15.6845

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Using zero-length covalent protein-DNA crosslinking, we have mapped the histone-DNA contacts in nucleosome core particles from which the C- and N-terminal domains of histone H2A were selectively trimmed by trypsin or clostripain. We found that the flexible trypsin-sensitive C-terminal domain of histone H2A contacts the dyad axis, whereas its globular domain contacts the end of DNA in the nucleosome core particle. The appearance of the histone H2A contact at the dyad axis occurs only in the absence of linker DNA and does not depend on the absence of linker histones. Our results show the ability of the histone H2A C-terminal domain to rearrange. This rearrangement might play a biological role in nucleosome disassembly and reassembly and the retention of the H2A-H2B dimer (or the whole octamer) during the passing of polymerases through the nucleosome.

Bibliography

Usachenko, S. I., Bavykin, S. G., Gavin, I. M., & Bradbury, E. M. (1994). Rearrangement of the histone H2A C-terminal domain in the nucleosome. Proceedings of the National Academy of Sciences, 91(15), 6845â6849.

Authors 4

S I Usachenko (first)
S G Bavykin (additional)
I M Gavin (additional)
E M Bradbury (additional)

References 0 Referenced 61

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:51 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:38 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 6, 2025, 9:42 a.m.)
Issued	31 years, 1 month ago (July 19, 1994)
Published	31 years, 1 month ago (July 19, 1994)
Published Online	31 years, 1 month ago (July 19, 1994)
Published Print	31 years, 1 month ago (July 19, 1994)

Funders 0

None

BibTeX

@article{Usachenko_1994, title={Rearrangement of the histone H2A C-terminal domain in the nucleosome.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.15.6845}, DOI={10.1073/pnas.91.15.6845}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Usachenko, S I and Bavykin, S G and Gavin, I M and Bradbury, E M}, year={1994}, month=jul, pages={6845–6849} }

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