DOI: 10.1073/pnas.91.14.6549. Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

10.1073/pnas.91.14.6549

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Myosin diversity in the human epithelial cell line Caco-2BBe, the porcine epithelial cell line LLC-PK1 (CL-4), human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded 8-11 putative myosins (depending on the cDNA source) representing six distinct myosin classes. Analysis of clones obtained by hybridization screening demonstrated that the original PCR products correspond to bona fide myosins, based on the presence of sequences highly conserved in other myosins. RNase protection analysis confirmed mRNA expression of 11 myosins in Caco-2BBe cells. Immunoblot analysis showed that at least 6 myosin immunogens are expressed in Caco-2BBe cells. The results reveal the existence of at least 11 unconventional human myosin genes, most of which are expressed in an overlapping fashion in different cell types. The abundance of myosins suggests that the myosin I vs. myosin II paradigm is inadequate to explain actin-based cellular motility.

Bibliography

Bement, W. M., Hasson, T., Wirth, J. A., Cheney, R. E., & Mooseker, M. S. (1994). Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types. Proceedings of the National Academy of Sciences, 91(14), 6549â6553.

Authors 5

W M Bement (first)
T Hasson (additional)
J A Wirth (additional)
R E Cheney (additional)
M S Mooseker (additional)

References 0 Referenced 111

None

Dates

Type	When
Created	19 years, 2 months ago (June 1, 2006, 8:34 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:43 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 7:30 a.m.)
Issued	31 years, 1 month ago (July 5, 1994)
Published	31 years, 1 month ago (July 5, 1994)
Published Online	31 years, 1 month ago (July 5, 1994)
Published Print	31 years, 1 month ago (July 5, 1994)

Funders 0

None

BibTeX

@article{Bement_1994, title={Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.14.6549}, DOI={10.1073/pnas.91.14.6549}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bement, W M and Hasson, T and Wirth, J A and Cheney, R E and Mooseker, M S}, year={1994}, month=jul, pages={6549–6553} }

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