DOI: 10.1073/pnas.91.13.5813. GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules.

GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules.

10.1073/pnas.91.13.5813

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The FtsZ protein is a GTPase that is essential for cell division in Escherichia coli. During cytokinesis, FtsZ localizes to a ring at the leading edge of septum synthesis. We report the GTP-dependent polymerization of purified FtsZ measured by sedimentation and light scattering. Electron microscopy of polymerized FtsZ revealed structures including tubules 14-20 nm in diameter with longitudinal arrays of protofilaments. FtsZ depolymerized upon removal of GTP and repolymerized after subsequent GTP addition. Mutant FtsZ84 protein polymerized inefficiently, suggesting that polymerization is important for the cellular role of FtsZ in division. The possibility that tubules of FtsZ protein form a cytoskeleton involved in septum synthesis is consistent with our data.

Bibliography

Bramhill, D., & Thompson, C. M. (1994). GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules. Proceedings of the National Academy of Sciences, 91(13), 5813â5817.

Authors 2

D Bramhill (first)
C M Thompson (additional)

References 0 Referenced 202

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:48 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:34 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	31 years, 2 months ago (June 21, 1994)
Published	31 years, 2 months ago (June 21, 1994)
Published Online	31 years, 2 months ago (June 21, 1994)
Published Print	31 years, 2 months ago (June 21, 1994)

Funders 0

None

BibTeX

@article{Bramhill_1994, title={GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.13.5813}, DOI={10.1073/pnas.91.13.5813}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bramhill, D and Thompson, C M}, year={1994}, month=jun, pages={5813–5817} }

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