DOI: 10.1073/pnas.91.10.4579. Similarity between a ubiquitous promoter element in an ancient eukaryote and mammalian initiator elements.

10.1073/pnas.91.10.4579

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

To identify regulatory elements that play a role in transcription initiation in ancient eukaryotes, we have analyzed the upstream regions of protein-coding genes from Trichomonas vaginalis, one of the most ancient eukaryotes studied to date. Characterization of seven protein-coding genes from this protist invariably revealed the presence of a highly conserved DNA sequence motif immediately upstream of the coding region. This 13-nt motif was shown to surround and contain precise sites for transcription initiation. No typical TATA boxes, positioned at 25-30 nt upstream of the transcription start sites of these genes, were found. The start-site regions from all seven T. vaginalis genes impart strong specific initiation of transcription in a mammalian in vitro transcription assay. This consensus promoter element in an ancient eukaryote is similar, both structurally and functionally, to initiator elements found in promoters of higher eukaryotes.

Bibliography

Quon, D. V., Delgadillo, M. G., Khachi, A., Smale, S. T., & Johnson, P. J. (1994). Similarity between a ubiquitous promoter element in an ancient eukaryote and mammalian initiator elements. Proceedings of the National Academy of Sciences, 91(10), 4579â4583.

Authors 5

D V Quon (first)
M G Delgadillo (additional)
A Khachi (additional)
S T Smale (additional)
P J Johnson (additional)

References 0 Referenced 58

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:46 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:41 p.m.)
Indexed	1 year, 2 months ago (May 31, 2024, 7:36 p.m.)
Issued	31 years, 3 months ago (May 10, 1994)
Published	31 years, 3 months ago (May 10, 1994)
Published Online	31 years, 3 months ago (May 10, 1994)
Published Print	31 years, 3 months ago (May 10, 1994)

Funders 0

None

BibTeX

@article{Quon_1994, title={Similarity between a ubiquitous promoter element in an ancient eukaryote and mammalian initiator elements.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.10.4579}, DOI={10.1073/pnas.91.10.4579}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Quon, D V and Delgadillo, M G and Khachi, A and Smale, S T and Johnson, P J}, year={1994}, month=may, pages={4579–4583} }

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