DOI: 10.1073/pnas.91.1.276. Direct measurement of forces between self-assembled proteins: temperature-dependent exponential forces between collagen triple helices.

Direct measurement of forces between self-assembled proteins: temperature-dependent exponential forces between collagen triple helices.

10.1073/pnas.91.1.276

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report direct measurements of force vs. separation between self-assembled proteins. These forces are observed between collagen triple helices in native and reconstituted fibers. They are a combination of a short-range repulsion, which varies exponentially over at least five decay lengths, and an inferred, longer-ranged attraction responsible for spontaneous assembly. From 5 degrees C to 35 degrees C the relative contribution of the attraction to the net force increases with temperature. These forces are strikingly similar to the "hydration" forces measured between several other linear macromolecules (DNA, polysaccharides) and between lipid bilayer membranes. The decay length of the repulsive force agrees well with a theoretical estimate based on axial periodicity of the triple helix, suggesting another connection between molecular architecture and protein-protein interaction.

Bibliography

Leikin, S., Rau, D. C., & Parsegian, V. A. (1994). Direct measurement of forces between self-assembled proteins: temperature-dependent exponential forces between collagen triple helices. Proceedings of the National Academy of Sciences, 91(1), 276â280.

Authors 3

S Leikin (first)
D C Rau (additional)
V A Parsegian (additional)

References 0 Referenced 125

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:37 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:26 p.m.)
Issued	31 years, 7 months ago (Jan. 4, 1994)
Published	31 years, 7 months ago (Jan. 4, 1994)
Published Online	31 years, 7 months ago (Jan. 4, 1994)
Published Print	31 years, 7 months ago (Jan. 4, 1994)

Funders 0

None

BibTeX

@article{Leikin_1994, title={Direct measurement of forces between self-assembled proteins: temperature-dependent exponential forces between collagen triple helices.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.1.276}, DOI={10.1073/pnas.91.1.276}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Leikin, S and Rau, D C and Parsegian, V A}, year={1994}, month=jan, pages={276–280} }

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