DOI: 10.1073/pnas.90.7.2608. Mycobacterium tuberculosis expresses two chaperonin-60 homologs.

Mycobacterium tuberculosis expresses two chaperonin-60 homologs.

10.1073/pnas.90.7.2608

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A 65-kDa protein and a 10-kDa protein are two of the more strongly immunoreactive components of Mycobacterium tuberculosis, the causative agent of tuberculosis. The 65-kDa antigen has homology with members of the GroEL or chaperonin-60 (Cpn60) family of heat shock proteins. The 10-kDa antigen has homology with the GroES or chaperonin-10 family of heat shock proteins. These two proteins are encoded by separate genes in M. tuberculosis. The studies reported here reveal that M. tuberculosis contains a second Cpn60 homolog located 98 bp downstream of the 10-kDa antigen gene. The second Cpn60 homolog (Cpn60-1) displays 61% amino acid sequence identity with the 65-kDa antigen (Cpn60-2) and 53% and 41% identity with the Escherichia coli GroEL protein and the human P60 protein, respectively. Primer-extension analysis revealed that transcription starts 29 bp upstream of the translation start of the Cpn60-1 homolog and protein purification studies indicate that the cpn60-1 gene is expressed as an approximately 60-kDa polypeptide.

Bibliography

Kong, T. H., Coates, A. R., Butcher, P. D., Hickman, C. J., & Shinnick, T. M. (1993). Mycobacterium tuberculosis expresses two chaperonin-60 homologs. Proceedings of the National Academy of Sciences, 90(7), 2608â2612.

Authors 5

T H Kong (first)
A R Coates (additional)
P D Butcher (additional)
C J Hickman (additional)
T M Shinnick (additional)

References 0 Referenced 116

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:48 p.m.)
Indexed	2 days, 18 hours ago (Aug. 29, 2025, 6:10 a.m.)
Issued	32 years, 5 months ago (April 1, 1993)
Published	32 years, 5 months ago (April 1, 1993)
Published Online	32 years, 5 months ago (April 1, 1993)
Published Print	32 years, 5 months ago (April 1, 1993)

Funders 0

None

BibTeX

@article{Kong_1993, title={Mycobacterium tuberculosis expresses two chaperonin-60 homologs.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.7.2608}, DOI={10.1073/pnas.90.7.2608}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kong, T H and Coates, A R and Butcher, P D and Hickman, C J and Shinnick, T M}, year={1993}, month=apr, pages={2608–2612} }

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