DOI: 10.1073/pnas.90.5.1671. Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.

Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.

10.1073/pnas.90.5.1671

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have expressed the recombinant squid kinesin head domain in Escherichia coli and studied its interaction with microtubules. The head is active as a microtubule-stimulated ATPase and binds to microtubules, but it does not support microtubule gliding by itself. The head binds to both microtubules and depolymerized tubulin. In each case the zero-length crosslinker 1-ethyl-3-[3-dimethylamino)propyl] carbodiimide induces a bond specifically to beta- but not alpha-tubulin. The head decorates brain microtubules with an 8-nm axial spacing. Thus the stoichiometry is one kinesin head per tubulin dimer. The lattice is that of flagellar B-tubules, implying that reassembled microtubules are not symmetric. Moreover, the A- and B-tubules of intact flagellar outer doublets are both decorated with a B lattice. This suggests that the B lattice is a general property of microtubules.

Bibliography

Song, Y. H., & Mandelkow, E. (1993). Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice. Proceedings of the National Academy of Sciences, 90(5), 1671â1675.

Authors 2

Y H Song (first)
E Mandelkow (additional)

References 0 Referenced 105

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:32 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	32 years, 6 months ago (March 1, 1993)
Published	32 years, 6 months ago (March 1, 1993)
Published Online	32 years, 6 months ago (March 1, 1993)
Published Print	32 years, 6 months ago (March 1, 1993)

Funders 0

None

BibTeX

@article{Song_1993, title={Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.5.1671}, DOI={10.1073/pnas.90.5.1671}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Song, Y H and Mandelkow, E}, year={1993}, month=mar, pages={1671–1675} }

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