DOI: 10.1073/pnas.90.3.843. Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.

Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.

10.1073/pnas.90.3.843

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Human RNA polymerase II is shown to be associated with a 3'-->5' exonuclease activity that removes nucleoside 5'-monophosphates from the 3' end of the transcripts in isolated ternary complexes. This activity is stimulated by SII, a protein that acts as a transcription elongation factor in vitro. In addition, we show that another transcription factor, TFIIF, stimulates a competing pyrophosphorolysis reaction. These findings raise interesting questions about the roles of these activities in vivo, including the possibility that this RNA polymerase may proofread the nascent transcript.

Bibliography

Wang, D., & Hawley, D. K. (1993). Identification of a 3â-->5â exonuclease activity associated with human RNA polymerase II. Proceedings of the National Academy of Sciences, 90(3), 843â847.

Authors 2

D Wang (first)
D K Hawley (additional)

References 0 Referenced 79

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:42 p.m.)
Indexed	1 month, 1 week ago (July 11, 2025, 6:31 a.m.)
Issued	32 years, 6 months ago (Feb. 1, 1993)
Published	32 years, 6 months ago (Feb. 1, 1993)
Published Online	32 years, 6 months ago (Feb. 1, 1993)
Published Print	32 years, 6 months ago (Feb. 1, 1993)

Funders 0

None

BibTeX

@article{Wang_1993, title={Identification of a 3’-->5’ exonuclease activity associated with human RNA polymerase II.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.3.843}, DOI={10.1073/pnas.90.3.843}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, D and Hawley, D K}, year={1993}, month=feb, pages={843–847} }

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