DOI: 10.1073/pnas.90.21.10140. Stimulation of Trypanosoma cruzi adenylyl cyclase by an alpha D-globin fragment from Triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms.

Stimulation of Trypanosoma cruzi adenylyl cyclase by an alpha D-globin fragment from Triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms.

10.1073/pnas.90.21.10140

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A peptide from hindguts of the Triatoma hematophagous Chagas insect vector activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes to metacyclic trypomastigotes. Hindguts were obtained from insects fed 2 days earlier with chicken blood. Purification was performed by gel filtration and HPLC on C18 and C4 columns. SDS/PAGE of the purified peptide showed a single band of about 10 kDa. The following sequence was determined for the 20 amino-terminal residues of this peptide: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln- Gln-Ala-Trp-Glu-Lys-Ala-Ala-Ser-His. This sequence is identical to the amino terminus of chicken alpha D-globin. On a Western blot, the peptide immunoreacted with a polyclonal antibody against chicken globin D. A synthetic peptide corresponding to residues 1-40 of the alpha D-globin amino terminus also stimulated adenylyl cyclase activity and promoted differentiation. This 125I-labeled synthetic peptide bound specifically to T. cruzi epimastigote cells. Activation of epimastigote adenylyl cyclase by the hemoglobin-derived peptide may play an important role in T. cruzi differentiation and consequently in the transmission of Chagas disease.

Bibliography

Fraidenraich, D., PeÃ±a, C., Isola, E. L., Lammel, E. M., Coso, O., AÃ±el, A. D., Pongor, S., Baralle, F., Torres, H. N., & Flawia, M. M. (1993). Stimulation of Trypanosoma cruzi adenylyl cyclase by an alpha D-globin fragment from Triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms. Proceedings of the National Academy of Sciences, 90(21), 10140â10144.

Authors 10

D Fraidenraich (first)
C Peña (additional)
E L Isola (additional)
E M Lammel (additional)
O Coso (additional)
A D Añel (additional)
S Pongor (additional)
F Baralle (additional)
H N Torres (additional)
M M Flawia (additional)

References 0 Referenced 65

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:34 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:30 p.m.)
Indexed	1 year, 2 months ago (June 6, 2024, 5:13 p.m.)
Issued	31 years, 10 months ago (Nov. 1, 1993)
Published	31 years, 10 months ago (Nov. 1, 1993)
Published Online	31 years, 10 months ago (Nov. 1, 1993)
Published Print	31 years, 10 months ago (Nov. 1, 1993)

Funders 0

None

BibTeX

@article{Fraidenraich_1993, title={Stimulation of Trypanosoma cruzi adenylyl cyclase by an alpha D-globin fragment from Triatoma hindgut: effect on differentiation of epimastigote to trypomastigote forms.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.21.10140}, DOI={10.1073/pnas.90.21.10140}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fraidenraich, D and Peña, C and Isola, E L and Lammel, E M and Coso, O and Añel, A D and Pongor, S and Baralle, F and Torres, H N and Flawia, M M}, year={1993}, month=nov, pages={10140–10144} }

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