DOI: 10.1073/pnas.90.2.427. Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector.

Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector.

10.1073/pnas.90.2.427

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

alpha-Trichosanthin, a eukaryotic ribosome-inactivating protein from Trichosanthes kirilowii, inhibits the replication of the human immunodeficiency virus (HIV) in vitro. The alpha-trichosanthin gene was placed under the transcriptional control of a tobamovirus subgenomic promoter in a plant RNA viral vector. Two weeks after inoculation, transfected Nicotiana benthamiana plants accumulated alpha-trichosanthin to levels of at least 2% of total soluble protein. The recombinant alpha-trichosanthin was purified and its structural and biological properties were analyzed. The 23-amino acid signal peptide was recognized by N. benthamiana and the processed enzyme caused a concentration-dependent inhibition of protein synthesis in vitro. The high level of heterologous gene expression observed in these studies is due to the unique features of the RNA viral-based transfection system.

Bibliography

Kumagai, M. H., Turpen, T. H., Weinzettl, N., della-Cioppa, G., Turpen, A. M., Donson, J., Hilf, M. E., Grantham, G. L., Dawson, W. O., & Chow, T. P. (1993). Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector. Proceedings of the National Academy of Sciences, 90(2), 427â430.

Authors 10

M H Kumagai (first)
T H Turpen (additional)
N Weinzettl (additional)
G della-Cioppa (additional)
A M Turpen (additional)
J Donson (additional)
M E Hilf (additional)
G L Grantham (additional)
W O Dawson (additional)
T P Chow (additional)

References 0 Referenced 116

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:48 p.m.)
Indexed	4 months, 3 weeks ago (April 9, 2025, 1:40 a.m.)
Issued	32 years, 7 months ago (Jan. 15, 1993)
Published	32 years, 7 months ago (Jan. 15, 1993)
Published Online	32 years, 7 months ago (Jan. 15, 1993)
Published Print	32 years, 7 months ago (Jan. 15, 1993)

Funders 0

None

BibTeX

@article{Kumagai_1993, title={Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.2.427}, DOI={10.1073/pnas.90.2.427}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kumagai, M H and Turpen, T H and Weinzettl, N and della-Cioppa, G and Turpen, A M and Donson, J and Hilf, M E and Grantham, G L and Dawson, W O and Chow, T P}, year={1993}, month=jan, pages={427–430} }

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