DOI: 10.1073/pnas.90.2.418. Use of a reporter transgene to generate arabidopsis mutants in ubiquitin-dependent protein degradation.

Use of a reporter transgene to generate arabidopsis mutants in ubiquitin-dependent protein degradation.

10.1073/pnas.90.2.418

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Ubiquitin-dependent proteolysis is a major proteolytic pathway in the cytoplasm and nucleus of eukaryotic cells. We introduced a gene encoding a substrate for this pathway into the genome of Arabidopsis thaliana. The transgene codes for a hybrid protein consisting of dihydrofolate reductase (DHFR, EC 1.5.1.3) fused to a degradation signal that is specifically recognized by components of the ubiquitin-dependent proteolysis pathway. Elevated concentrations of the DHFR protein confer resistance to the drug methotrexate, but rapid degradation prevents accumulation of the protein in the plant. Therefore, transgenic A. thaliana lines expressing the DHFR fusion protein are methotrexate-sensitive. Selection for mutants resistant to methotrexate resulted in plants impaired in degradation of the DHFR model substrate, as shown by an increase in protein level in the mutants.

Bibliography

Bachmair, A., Becker, F., & Schell, J. (1993). Use of a reporter transgene to generate arabidopsis mutants in ubiquitin-dependent protein degradation. Proceedings of the National Academy of Sciences, 90(2), 418â421.

Authors 3

A Bachmair (first)
F Becker (additional)
J Schell (additional)

References 0 Referenced 38

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:47 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 5, 2025, 9:03 a.m.)
Issued	32 years, 7 months ago (Jan. 15, 1993)
Published	32 years, 7 months ago (Jan. 15, 1993)
Published Online	32 years, 7 months ago (Jan. 15, 1993)
Published Print	32 years, 7 months ago (Jan. 15, 1993)

Funders 0

None

BibTeX

@article{Bachmair_1993, title={Use of a reporter transgene to generate arabidopsis mutants in ubiquitin-dependent protein degradation.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.2.418}, DOI={10.1073/pnas.90.2.418}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bachmair, A and Becker, F and Schell, J}, year={1993}, month=jan, pages={418–421} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T13:03:20Z",
    "timestamp": 1754399000053
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "2",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        1,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Ubiquitin-dependent proteolysis is a major proteolytic pathway in the cytoplasm and nucleus of eukaryotic cells. We introduced a gene encoding a substrate for this pathway into the genome of Arabidopsis thaliana. The transgene codes for a hybrid protein consisting of dihydrofolate reductase (DHFR, EC 1.5.1.3) fused to a degradation signal that is specifically recognized by components of the ubiquitin-dependent proteolysis pathway. Elevated concentrations of the DHFR protein confer resistance to the drug methotrexate, but rapid degradation prevents accumulation of the protein in the plant. Therefore, transgenic A. thaliana lines expressing the DHFR fusion protein are methotrexate-sensitive. Selection for mutants resistant to methotrexate resulted in plants impaired in degradation of the DHFR model substrate, as shown by an increase in protein level in the mutants.</jats:p>",
  "DOI": "10.1073/pnas.90.2.418",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T12:32:43Z",
    "timestamp": 1149078763000
  },
  "page": "418-421",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 38,
  "title": "Use of a reporter transgene to generate arabidopsis mutants in ubiquitin-dependent protein degradation.",
  "prefix": "10.1073",
  "volume": "90",
  "author": [
    {
      "given": "A",
      "family": "Bachmair",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Cologne, Federal Republic of Germany."
        }
      ]
    },
    {
      "given": "F",
      "family": "Becker",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Cologne, Federal Republic of Germany."
        }
      ]
    },
    {
      "given": "J",
      "family": "Schell",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Cologne, Federal Republic of Germany."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1993,
        1,
        15
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.90.2.418",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:47:55Z",
    "timestamp": 1649872075000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.90.2.418"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        1,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1993,
          1,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.90.2.418"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.90.2.418",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1993,
        1,
        15
      ]
    ]
  },
  "assertion": [
    {
      "value": "1993-01-15",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}