DOI: 10.1073/pnas.90.18.8444. Physical interaction between the herpes simplex virus 1 origin-binding protein and single-stranded DNA-binding protein ICP8.

Physical interaction between the herpes simplex virus 1 origin-binding protein and single-stranded DNA-binding protein ICP8.

10.1073/pnas.90.18.8444

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We had previously demonstrated that the herpes simplex virus 1 (HSV-1) single-stranded DNA-binding protein (ICP8) can specifically stimulate the helicase activity of the HSV-1 origin-binding protein (UL9). We show here that this functional stimulation is a manifestation of a tight interaction between UL9 protein and ICP8. By using protein-affinity chromatography, we have demonstrated the specific binding of purified UL9 protein to immobilized ICP8 and vice versa. Furthermore, ICP8 is specifically retained by a column on which the C-terminal 37-kDa DNA-binding domain of the UL9 protein was immobilized. The interaction between ICP8 and the DNA-binding domain of the UL9 protein was confirmed by cochromatography of the two proteins. These results suggest that the UL9 protein and ICP8 form a tight complex that functions in origin recognition and unwinding.

Bibliography

Boehmer, P. E., & Lehman, I. R. (1993). Physical interaction between the herpes simplex virus 1 origin-binding protein and single-stranded DNA-binding protein ICP8. Proceedings of the National Academy of Sciences, 90(18), 8444â8448.

Authors 2

P E Boehmer (first)
I R Lehman (additional)

References 0 Referenced 80

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:37 p.m.)
Indexed	2 days, 5 hours ago (Aug. 28, 2025, 8:24 a.m.)
Issued	31 years, 11 months ago (Sept. 15, 1993)
Published	31 years, 11 months ago (Sept. 15, 1993)
Published Online	31 years, 11 months ago (Sept. 15, 1993)
Published Print	31 years, 11 months ago (Sept. 15, 1993)

Funders 0

None

BibTeX

@article{Boehmer_1993, title={Physical interaction between the herpes simplex virus 1 origin-binding protein and single-stranded DNA-binding protein ICP8.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.18.8444}, DOI={10.1073/pnas.90.18.8444}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Boehmer, P E and Lehman, I R}, year={1993}, month=sep, pages={8444–8448} }

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