DOI: 10.1073/pnas.90.13.6125. Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.

Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.

10.1073/pnas.90.13.6125

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral RNA and DNA in association with viral matrix (MA), integrase (IN), and reverse transcriptase (RT) antigens but not capsid (CA) antigens and possessed integration activity in vitro. Association of IN but not RT or MA antigens with viral DNA was detergent-stable. Analysis of viral DNA synthesis and nuclear import of viral nucleoprotein complexes in the presence of a reversible RT inhibitor demonstrated that reverse transcription of viral RNA could be completed entirely in the host cell nucleus. Our studies demonstrate structural and functional features of the nucleoprotein (preintegration) complex of HIV-1 which are pertinent to the understanding of early events in the lentiviral life cycle.

Bibliography

Bukrinsky, M. I., Sharova, N., McDonald, T. L., Pushkarskaya, T., Tarpley, W. G., & Stevenson, M. (1993). Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection. Proceedings of the National Academy of Sciences, 90(13), 6125â6129.

Authors 6

M I Bukrinsky (first)
N Sharova (additional)
T L McDonald (additional)
T Pushkarskaya (additional)
W G Tarpley (additional)
M Stevenson (additional)

References 0 Referenced 344

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:25 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:26 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 3, 2025, 7:04 p.m.)
Issued	32 years, 1 month ago (July 1, 1993)
Published	32 years, 1 month ago (July 1, 1993)
Published Online	32 years, 1 month ago (July 1, 1993)
Published Print	32 years, 1 month ago (July 1, 1993)

Funders 0

None

BibTeX

@article{Bukrinsky_1993, title={Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.13.6125}, DOI={10.1073/pnas.90.13.6125}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bukrinsky, M I and Sharova, N and McDonald, T L and Pushkarskaya, T and Tarpley, W G and Stevenson, M}, year={1993}, month=jul, pages={6125–6129} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        3
      ]
    ],
    "date-time": "2025-08-03T23:04:35Z",
    "timestamp": 1754262275506
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "13",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        7
      ]
    ]
  },
  "abstract": "<jats:p>We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral RNA and DNA in association with viral matrix (MA), integrase (IN), and reverse transcriptase (RT) antigens but not capsid (CA) antigens and possessed integration activity in vitro. Association of IN but not RT or MA antigens with viral DNA was detergent-stable. Analysis of viral DNA synthesis and nuclear import of viral nucleoprotein complexes in the presence of a reversible RT inhibitor demonstrated that reverse transcription of viral RNA could be completed entirely in the host cell nucleus. Our studies demonstrate structural and functional features of the nucleoprotein (preintegration) complex of HIV-1 which are pertinent to the understanding of early events in the lentiviral life cycle.</jats:p>",
  "DOI": "10.1073/pnas.90.13.6125",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T12:25:50Z",
    "timestamp": 1149078350000
  },
  "page": "6125-6129",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 344,
  "title": "Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.",
  "prefix": "10.1073",
  "volume": "90",
  "author": [
    {
      "given": "M I",
      "family": "Bukrinsky",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    },
    {
      "given": "N",
      "family": "Sharova",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    },
    {
      "given": "T L",
      "family": "McDonald",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    },
    {
      "given": "T",
      "family": "Pushkarskaya",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    },
    {
      "given": "W G",
      "family": "Tarpley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    },
    {
      "given": "M",
      "family": "Stevenson",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1993,
        7
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.90.13.6125",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:26:33Z",
    "timestamp": 1649870793000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.90.13.6125"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        7
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "13",
    "published-print": {
      "date-parts": [
        [
          1993,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.90.13.6125"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.90.13.6125",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1993,
        7
      ]
    ]
  },
  "assertion": [
    {
      "value": "1993-07-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}