DOI: 10.1073/pnas.90.12.5732. A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus.

A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus.

10.1073/pnas.90.12.5732

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A soluble secretory protein is usually present at a much higher concentration in the Golgi apparatus than in the endoplasmic reticulum (ER) inside eukaryotic secretory cells in the steady state. We show by immunoelectron microscopic experiments with the soluble secretory protein serum albumin, inside Hep-G2 human hepatoma cells in culture, that the secretory protein is first concentrated at isolated sites within the ER before it is transferred to the cis face of the Golgi apparatus. This is contrary to expectations of the bulk-flow hypothesis of ER-to-Golgi transfer, and it suggests the involvement of concentration and transfer mechanisms within the ER that have not previously been recognized.

Bibliography

Mizuno, M., & Singer, S. J. (1993). A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus. Proceedings of the National Academy of Sciences, 90(12), 5732â5736.

Authors 2

M Mizuno (first)
S J Singer (additional)

References 0 Referenced 86

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:25 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:45 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 8:21 a.m.)
Issued	32 years, 2 months ago (June 15, 1993)
Published	32 years, 2 months ago (June 15, 1993)
Published Online	32 years, 2 months ago (June 15, 1993)
Published Print	32 years, 2 months ago (June 15, 1993)

Funders 0

None

BibTeX

@article{Mizuno_1993, title={A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus.}, volume={90}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.90.12.5732}, DOI={10.1073/pnas.90.12.5732}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mizuno, M and Singer, S J}, year={1993}, month=jun, pages={5732–5736} }

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