DOI: 10.1073/pnas.89.5.1557. Role of the alpha v beta 3 integrin in human melanoma cell invasion.

Role of the alpha v beta 3 integrin in human melanoma cell invasion.

10.1073/pnas.89.5.1557

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The human melanoma cell line A375M expresses the vitronectin receptor (alpha v beta 3 integrin) on its cell surface. Treatment of A375M cells with either polyclonal or monoclonal anti-alpha v beta 3 antibodies resulted in stimulation of invasion through basement membrane matrices in vitro. Similar treatment of these cells with a monoclonal anti-alpha v antibody, which does not inhibit the adhesive function of the alpha v beta 3 antigen, also stimulated invasion; however, anti-beta 3 antibody treatment had no effect. Furthermore, pretreatment of the cells with vitronectin or addition of vitronectin to the basement membrane matrix also resulted in stimulation of invasion. Similar treatments with fibronectin receptor antibody or fibronectin had no effect on invasion. Analysis of type IV collagenase expression in cells treated with anti-alpha v beta 3 antibody showed higher levels of both the secreted 72-kDa enzyme and its mRNA. Signal transduction through the alpha v beta 3 integrin could underlie the elevated expression of metalloproteinase and the enhanced invasion of A375M cells through basement membrane matrices.

Bibliography

Seftor, R. E., Seftor, E. A., Gehlsen, K. R., Stetler-Stevenson, W. G., Brown, P. D., Ruoslahti, E., & Hendrix, M. J. (1992). Role of the alpha v beta 3 integrin in human melanoma cell invasion. Proceedings of the National Academy of Sciences, 89(5), 1557â1561.

Authors 7

R E Seftor (first)
E A Seftor (additional)
K R Gehlsen (additional)
W G Stetler-Stevenson (additional)
P D Brown (additional)
E Ruoslahti (additional)
M J Hendrix (additional)

References 0 Referenced 313

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:17 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:43 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:43 p.m.)
Issued	33 years, 6 months ago (March 1, 1992)
Published	33 years, 6 months ago (March 1, 1992)
Published Online	33 years, 6 months ago (March 1, 1992)
Published Print	33 years, 6 months ago (March 1, 1992)

Funders 0

None

BibTeX

@article{Seftor_1992, title={Role of the alpha v beta 3 integrin in human melanoma cell invasion.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.5.1557}, DOI={10.1073/pnas.89.5.1557}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Seftor, R E and Seftor, E A and Gehlsen, K R and Stetler-Stevenson, W G and Brown, P D and Ruoslahti, E and Hendrix, M J}, year={1992}, month=mar, pages={1557–1561} }

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