DOI: 10.1073/pnas.89.22.10852. Assembly of a hetero-oligomeric membrane protein complex.

Assembly of a hetero-oligomeric membrane protein complex.

10.1073/pnas.89.22.10852

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The maltose transporter of Escherichia coli is a hetero-oligomeric complex located in the cytoplasmic membrane of the cell. The in vivo assembly of this complex has been examined by using an assay based on the proteolytic sensitivity of one of its components, MalF. Immediately after synthesis and insertion into the membrane, MalF is sensitive to exogenously added proteases. In a time- and complex assembly-dependent fashion, MalF becomes protease resistant. Using this assay, we show that MalF is inserted into the membrane independently of other components of the transport complex. The assembly of the maltose transport complex occurs subsequently from a pool of freely diffusing protein in the membrane. This assembly process is efficient and occurs with rapid kinetics.

Bibliography

Traxler, B., & Beckwith, J. (1992). Assembly of a hetero-oligomeric membrane protein complex. Proceedings of the National Academy of Sciences, 89(22), 10852â10856.

Authors 2

B Traxler (first)
J Beckwith (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:12 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:40 p.m.)
Indexed	1 month, 1 week ago (July 20, 2025, midnight)
Issued	32 years, 9 months ago (Nov. 15, 1992)
Published	32 years, 9 months ago (Nov. 15, 1992)
Published Online	32 years, 9 months ago (Nov. 15, 1992)
Published Print	32 years, 9 months ago (Nov. 15, 1992)

Funders 0

None

BibTeX

@article{Traxler_1992, title={Assembly of a hetero-oligomeric membrane protein complex.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.22.10852}, DOI={10.1073/pnas.89.22.10852}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Traxler, B and Beckwith, J}, year={1992}, month=nov, pages={10852–10856} }

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