DOI: 10.1073/pnas.89.21.10159. Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.

Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.

10.1073/pnas.89.21.10159

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.

Bibliography

Watanabe, T., Ono, Y., Taniyama, Y., Hazama, K., Igarashi, K., Ogita, K., Kikkawa, U., & Nishizuka, Y. (1992). Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies. Proceedings of the National Academy of Sciences, 89(21), 10159â10163.

Authors 8

T Watanabe (first)
Y Ono (additional)
Y Taniyama (additional)
K Hazama (additional)
K Igarashi (additional)
K Ogita (additional)
U Kikkawa (additional)
Y Nishizuka (additional)

References 0 Referenced 238

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:11 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:49 p.m.)
Indexed	1 day ago (Sept. 3, 2025, 6:21 a.m.)
Issued	32 years, 10 months ago (Nov. 1, 1992)
Published	32 years, 10 months ago (Nov. 1, 1992)
Published Online	32 years, 10 months ago (Nov. 1, 1992)
Published Print	32 years, 10 months ago (Nov. 1, 1992)

Funders 0

None

BibTeX

@article{Watanabe_1992, title={Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.21.10159}, DOI={10.1073/pnas.89.21.10159}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Watanabe, T and Ono, Y and Taniyama, Y and Hazama, K and Igarashi, K and Ogita, K and Kikkawa, U and Nishizuka, Y}, year={1992}, month=nov, pages={10159–10163} }

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