DOI: 10.1073/pnas.89.20.9875. A 105-kDa protein is required for yeast mitochondrial RNase P activity.

A 105-kDa protein is required for yeast mitochondrial RNase P activity.

10.1073/pnas.89.20.9875

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

RNase P from the mitochondria of Saccharomyces cerevisiae was purified to near homogeneity > 1800-fold with a yield of 1.6% from mitochondrial extracts. The most abundant protein in the purified fractions is, at 105 kDa, considerably larger than the 14-kDa bacterial RNase P protein subunits. Oligonucleotides designed from the amino-terminal sequence of the 105-kDa protein were used to identify and isolate the 105-kDa protein-encoding gene. Strains carrying a disruption of the gene for the 105-kDa protein are viable but respiratory deficient and accumulate mitochondrial tRNA precursors with 5' extensions. As this is the second gene known to be necessary for yeast mitochondrial RNase P activity, we have named it RPM2 (for RNase P mitochondrial).

Bibliography

Morales, M. J., Dang, Y. L., Lou, Y. C., Sulo, P., & Martin, N. C. (1992). A 105-kDa protein is required for yeast mitochondrial RNase P activity. Proceedings of the National Academy of Sciences, 89(20), 9875â9879.

Authors 5

M J Morales (first)
Y L Dang (additional)
Y C Lou (additional)
P Sulo (additional)
N C Martin (additional)

References 0 Referenced 73

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:10 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:30 p.m.)
Indexed	1 year ago (July 30, 2024, 1:16 p.m.)
Issued	32 years, 10 months ago (Oct. 15, 1992)
Published	32 years, 10 months ago (Oct. 15, 1992)
Published Online	32 years, 10 months ago (Oct. 15, 1992)
Published Print	32 years, 10 months ago (Oct. 15, 1992)

Funders 0

None

BibTeX

@article{Morales_1992, title={A 105-kDa protein is required for yeast mitochondrial RNase P activity.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.20.9875}, DOI={10.1073/pnas.89.20.9875}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Morales, M J and Dang, Y L and Lou, Y C and Sulo, P and Martin, N C}, year={1992}, month=oct, pages={9875–9879} }

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