DOI: 10.1073/pnas.89.20.9382. Nitric oxide stimulates auto-ADP-ribosylation of glyceraldehyde-3-phosphate dehydrogenase.

Nitric oxide stimulates auto-ADP-ribosylation of glyceraldehyde-3-phosphate dehydrogenase.

10.1073/pnas.89.20.9382

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Nitric oxide generation in brain cytosolic fractions markedly enhances ADP-ribosylation of a single 37-kDa protein. By utilizing a biotinylated NAD and avidin affinity chromatography, we purified this protein. Partial amino acid sequencing establishes its identity as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This is further confirmed by detection of GAPDH enzymatic activity in the purified 37-kDa protein. GAPDH is ADP-ribosylated in the absence of brain extract. This auto-ADP-ribosylation is enhanced by nitric oxide generation. ADP-ribosylation appears to involve the cysteine where NAD interacts with GAPDH so that ADP-ribosylation likely inhibits enzymatic activity. Such inhibition may play a role in nitric oxide-mediated neurotoxicity.

Bibliography

Zhang, J., & Snyder, S. H. (1992). Nitric oxide stimulates auto-ADP-ribosylation of glyceraldehyde-3-phosphate dehydrogenase. Proceedings of the National Academy of Sciences, 89(20), 9382â9385.

Authors 2

J Zhang (first)
S H Snyder (additional)

References 0 Referenced 201

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:10 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:31 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	32 years, 10 months ago (Oct. 15, 1992)
Published	32 years, 10 months ago (Oct. 15, 1992)
Published Online	32 years, 10 months ago (Oct. 15, 1992)
Published Print	32 years, 10 months ago (Oct. 15, 1992)

Funders 0

None

BibTeX

@article{Zhang_1992, title={Nitric oxide stimulates auto-ADP-ribosylation of glyceraldehyde-3-phosphate dehydrogenase.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.20.9382}, DOI={10.1073/pnas.89.20.9382}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhang, J and Snyder, S H}, year={1992}, month=oct, pages={9382–9385} }

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