DOI: 10.1073/pnas.89.18.8794. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

10.1073/pnas.89.18.8794

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.

Bibliography

Shizuya, H., Birren, B., Kim, U. J., Mancino, V., Slepak, T., Tachiiri, Y., & Simon, M. (1992). Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proceedings of the National Academy of Sciences, 89(18), 8794â8797.

Authors 7

H Shizuya (first)
B Birren (additional)
U J Kim (additional)
V Mancino (additional)
T Slepak (additional)
Y Tachiiri (additional)
M Simon (additional)

References 0 Referenced 1,229

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:08 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:25 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 4:41 p.m.)
Issued	32 years, 11 months ago (Sept. 15, 1992)
Published	32 years, 11 months ago (Sept. 15, 1992)
Published Online	32 years, 11 months ago (Sept. 15, 1992)
Published Print	32 years, 11 months ago (Sept. 15, 1992)

Funders 0

None

BibTeX

@article{Shizuya_1992, title={Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.18.8794}, DOI={10.1073/pnas.89.18.8794}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shizuya, H and Birren, B and Kim, U J and Mancino, V and Slepak, T and Tachiiri, Y and Simon, M}, year={1992}, month=sep, pages={8794–8797} }

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