DOI: 10.1073/pnas.89.18.8769. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.

Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.

10.1073/pnas.89.18.8769

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

Bibliography

Zhang, M., Zamore, P. D., Carmo-Fonseca, M., Lamond, A. I., & Green, M. R. (1992). Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proceedings of the National Academy of Sciences, 89(18), 8769â8773.

Authors 5

M Zhang (first)
P D Zamore (additional)
M Carmo-Fonseca (additional)
A I Lamond (additional)
M R Green (additional)

References 0 Referenced 142

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:08 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:26 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	32 years, 11 months ago (Sept. 15, 1992)
Published	32 years, 11 months ago (Sept. 15, 1992)
Published Online	32 years, 11 months ago (Sept. 15, 1992)
Published Print	32 years, 11 months ago (Sept. 15, 1992)

Funders 0

None

BibTeX

@article{Zhang_1992, title={Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.18.8769}, DOI={10.1073/pnas.89.18.8769}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhang, M and Zamore, P D and Carmo-Fonseca, M and Lamond, A I and Green, M R}, year={1992}, month=sep, pages={8769–8773} }

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