DOI: 10.1073/pnas.89.18.8651. Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.

Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.

10.1073/pnas.89.18.8651

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Escherichia coli able to specifically adhere to iron oxide and not adhere to other metal oxides were constructed by genetic engineering. Concatamers of random oligonucleotides were introduced into a portion of a plasmid-borne lamB gene encoding an external domain of the phage lambda receptor. Bacteria able to adhere to iron oxide were selected by serial enrichment from the population of plasmid transformants. The concatameric nature of the inserted DNA allows a genetic analysis analogous to exons shuffling. Results of this genetic analysis indicate that in some isolates, part of the binding site is encoded by flanking vector sequences. This strategy may prove generally useful for identifying protein sequences able to recognize specific surfaces.

Bibliography

Brown, S. (1992). Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Proceedings of the National Academy of Sciences, 89(18), 8651â8655.

Authors 1

S Brown (first)

References 0 Referenced 211

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:08 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:26 p.m.)
Indexed	3 months, 1 week ago (May 21, 2025, 12:27 a.m.)
Issued	32 years, 11 months ago (Sept. 15, 1992)
Published	32 years, 11 months ago (Sept. 15, 1992)
Published Online	32 years, 11 months ago (Sept. 15, 1992)
Published Print	32 years, 11 months ago (Sept. 15, 1992)

Funders 0

None

BibTeX

@article{Brown_1992, title={Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.18.8651}, DOI={10.1073/pnas.89.18.8651}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Brown, S}, year={1992}, month=sep, pages={8651–8655} }

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