DOI: 10.1073/pnas.89.14.6232. Targeted oncogene activation by site-specific recombination in transgenic mice.

Targeted oncogene activation by site-specific recombination in transgenic mice.

10.1073/pnas.89.14.6232

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.

Bibliography

Lakso, M., Sauer, B., Mosinger, B., Lee, E. J., Manning, R. W., Yu, S. H., Mulder, K. L., & Westphal, H. (1992). Targeted oncogene activation by site-specific recombination in transgenic mice. Proceedings of the National Academy of Sciences, 89(14), 6232â6236.

Authors 8

M Lakso (first)
B Sauer (additional)
B Mosinger (additional)
E J Lee (additional)
R W Manning (additional)
S H Yu (additional)
K L Mulder (additional)
H Westphal (additional)

References 0 Referenced 502

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 8:05 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:02 p.m.)
Indexed	7 hours, 57 minutes ago (Aug. 29, 2025, 6:08 a.m.)
Issued	33 years, 1 month ago (July 15, 1992)
Published	33 years, 1 month ago (July 15, 1992)
Published Online	33 years, 1 month ago (July 15, 1992)
Published Print	33 years, 1 month ago (July 15, 1992)

Funders 0

None

BibTeX

@article{Lakso_1992, title={Targeted oncogene activation by site-specific recombination in transgenic mice.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.14.6232}, DOI={10.1073/pnas.89.14.6232}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lakso, M and Sauer, B and Mosinger, B and Lee, E J and Manning, R W and Yu, S H and Mulder, K L and Westphal, H}, year={1992}, month=jul, pages={6232–6236} }

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