DOI: 10.1073/pnas.89.12.5447. Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.

Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.

10.1073/pnas.89.12.5447

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The double-stranded RNA (dsRNA)-binding domain of the human p68 kinase has been localized to the N-terminal half of the enzyme by using progressive deletion analysis and in vitro binding assays. To further define the domains responsible for binding to dsRNA, we cloned the mouse dsRNA-activated p65 kinase and used sequence alignment to identify conserved domains in the N-terminal region. Deletions in either of two 12-amino-acid-long and arginine- or lysine-rich regions abrogated binding to dsRNA. Moreover, in an in vivo growth inhibition assay in the yeast Saccharomyces cerevisiae, these mutants failed to exhibit a slow-growth phenotype.

Bibliography

Feng, G. S., Chong, K., Kumar, A., & Williams, B. R. (1992). Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase. Proceedings of the National Academy of Sciences, 89(12), 5447â5451.

Authors 4

G S Feng (first)
K Chong (additional)
A Kumar (additional)
B R Williams (additional)

References 0 Referenced 139

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 8:02 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:02 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:40 a.m.)
Issued	33 years, 2 months ago (June 15, 1992)
Published	33 years, 2 months ago (June 15, 1992)
Published Online	33 years, 2 months ago (June 15, 1992)
Published Print	33 years, 2 months ago (June 15, 1992)

Funders 0

None

BibTeX

@article{Feng_1992, title={Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.12.5447}, DOI={10.1073/pnas.89.12.5447}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Feng, G S and Chong, K and Kumar, A and Williams, B R}, year={1992}, month=jun, pages={5447–5451} }

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