DOI: 10.1073/pnas.89.1.28. Yeast casein kinase I homologues: an essential gene pair.

Yeast casein kinase I homologues: an essential gene pair.

10.1073/pnas.89.1.28

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report the isolation of an essential pair of Saccharomyces cerevisiae genes that encode protein kinase homologues. The two genes were independently isolated as dosage-dependent suppressors. Increased dosage of YCK1 suppressed defects caused by reduced SNF1 protein kinase activity, and increased dosage of YCK2 relieved sensitivity of wild-type cells to salt stress. The two genes function identically in the two growth assays, and loss of function of either gene alone has no discernible effect on growth. However, loss of function of both genes results in inviability. The two predicted protein products share 77% overall amino acid identity and contain sequence elements conserved among protein kinases. Partial sequence obtained for rabbit casein kinase I shares 64% identity with the two yeast gene products. Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues.

Bibliography

Robinson, L. C., Hubbard, E. J., Graves, P. R., DePaoli-Roach, A. A., Roach, P. J., Kung, C., Haas, D. W., Hagedorn, C. H., Goebl, M., & Culbertson, M. R. (1992). Yeast casein kinase I homologues: an essential gene pair. Proceedings of the National Academy of Sciences, 89(1), 28â32.

Authors 10

L C Robinson (first)
E J Hubbard (additional)
P R Graves (additional)
A A DePaoli-Roach (additional)
P J Roach (additional)
C Kung (additional)
D W Haas (additional)
C H Hagedorn (additional)
M Goebl (additional)
M R Culbertson (additional)

References 0 Referenced 115

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:59 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:08 p.m.)
Indexed	1 month, 4 weeks ago (July 4, 2025, 7:48 a.m.)
Issued	33 years, 8 months ago (Jan. 1, 1992)
Published	33 years, 8 months ago (Jan. 1, 1992)
Published Online	33 years, 8 months ago (Jan. 1, 1992)
Published Print	33 years, 8 months ago (Jan. 1, 1992)

Funders 0

None

BibTeX

@article{Robinson_1992, title={Yeast casein kinase I homologues: an essential gene pair.}, volume={89}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.89.1.28}, DOI={10.1073/pnas.89.1.28}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Robinson, L C and Hubbard, E J and Graves, P R and DePaoli-Roach, A A and Roach, P J and Kung, C and Haas, D W and Hagedorn, C H and Goebl, M and Culbertson, M R}, year={1992}, month=jan, pages={28–32} }

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