DOI: 10.1073/pnas.88.9.4045. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.

The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.

10.1073/pnas.88.9.4045

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The mechanism of Tat transactivation was studied by treating cell lines containing Tat-defective viruses with purified Tat protein. These cell lines constitutively produce very low levels of virus in the absence of Tat, as measured by p24 antigen levels. Virus production can be increased greater than 30,000-fold by adding exogenous Tat. Tat addition increases mRNA levels early in the viral life cycle, and Tat is required for Rev function to become evident. There is no evidence for a translational effect of Tat. Nuclear run-on experiments show that the increase in mRNA levels is due to an increased efficiency of elongation of nascent transcripts. These results suggest that Tat may be a gene-specific elongation factor.

Authors 3

M B Feinberg (first)
D Baltimore (additional)
A D Frankel (additional)

References 0 Referenced 278

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:58 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:21 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:45 p.m.)
Issued	34 years, 3 months ago (May 1, 1991)
Published	34 years, 3 months ago (May 1, 1991)
Published Online	34 years, 3 months ago (May 1, 1991)
Published Print	34 years, 3 months ago (May 1, 1991)

Funders 0

None

BibTeX

@article{Feinberg_1991, title={The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.9.4045}, DOI={10.1073/pnas.88.9.4045}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Feinberg, M B and Baltimore, D and Frankel, A D}, year={1991}, month=may, pages={4045–4049} }

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