DOI: 10.1073/pnas.88.9.3559. Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.

Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.

10.1073/pnas.88.9.3559

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

High-level expression of the full-length human retinoic acid receptor (RAR) alpha and the DNA binding domain of the RAR in Escherichia coli was achieved by using a T7 RNA polymerase-directed expression system. After induction, full-length RAR protein was produced at an estimated level of 20% of the total bacterial proteins. Both intact RAR molecules and the DNA binding domain bind to the cognate DNA response element with high specificity in the absence of retinoic acid. However, this binding is enhanced to a great extent upon the addition of eukaryotic cell extracts. The factor responsible for this enhancement is heat-sensitive and forms a complex with RAR that binds to DNA and exhibits a distinct migration pattern in the gel-mobility-shift assay. The interaction site of the factor with RAR is localized in the 70-amino acid DNA binding region of RAR. The hormone binding ability of the RAR alpha protein was assayed by a charcoal absorption assay and the RAR protein was found to bind to retinoic acid with a Kd of 2.1 x 10(-10) M.

Bibliography

Yang, N., SchÃ¼le, R., Mangelsdorf, D. J., & Evans, R. M. (1991). Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor. Proceedings of the National Academy of Sciences, 88(9), 3559â3563.

Authors 4

N Yang (first)
R Schüle (additional)
D J Mangelsdorf (additional)
R M Evans (additional)

References 0 Referenced 69

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:58 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:21 p.m.)
Indexed	1 year, 6 months ago (Feb. 26, 2024, 3:16 a.m.)
Issued	34 years, 4 months ago (May 1, 1991)
Published	34 years, 4 months ago (May 1, 1991)
Published Online	34 years, 4 months ago (May 1, 1991)
Published Print	34 years, 4 months ago (May 1, 1991)

Funders 0

None

BibTeX

@article{Yang_1991, title={Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.9.3559}, DOI={10.1073/pnas.88.9.3559}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yang, N and Schüle, R and Mangelsdorf, D J and Evans, R M}, year={1991}, month=may, pages={3559–3563} }

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