DOI: 10.1073/pnas.88.7.2687. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.

Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.

10.1073/pnas.88.7.2687

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The SNF2, SNF5, and SNF6 genes of Saccharomyces cerevisiae are required for expression of a variety of differently regulated genes. Previous evidence implicated the SNF5 protein in transcriptional activation, and a DNA-bound LexA-SNF5 fusion protein was shown to activate expression of a nearby promoter. Here, we examine the functional relationship of the SNF2, SNF5, and SNF6 proteins. Activation by DNA-bound LexA-SNF5 fusion protein was greatly reduced in snf2 and snf6 mutants, indicating that activation by LexA-SNF5 requires SNF2 and SNF6 function. An spt6 mutation, which suppresses transcriptional defects caused by snf2, restored activation by LexA-SNF5 in a snf2 mutant. The SNF2 gene was sequenced and encodes a 194-kDa protein that is targeted to the nucleus. DNA-bound LexA-SNF2 fusion protein also activated transcription, dependent on SNF5 and SNF6. These findings suggest that SNF2, SNF5, and SNF6 function interdependently in transcriptional activation, possibly forming a heteromeric complex.

Authors 3

B C Laurent (first)
M A Treitel (additional)
M Carlson (additional)

References 0 Referenced 170

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:56 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:04 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	34 years, 4 months ago (April 1, 1991)
Published	34 years, 4 months ago (April 1, 1991)
Published Online	34 years, 4 months ago (April 1, 1991)
Published Print	34 years, 4 months ago (April 1, 1991)

Funders 0

None

BibTeX

@article{Laurent_1991, title={Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.7.2687}, DOI={10.1073/pnas.88.7.2687}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Laurent, B C and Treitel, M A and Carlson, M}, year={1991}, month=apr, pages={2687–2691} }

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