DOI: 10.1073/pnas.88.6.2530. Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.

Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.

10.1073/pnas.88.6.2530

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to previous data obtained with other murine erythroleukemia cells, we detected activation of human chromosomal gamma-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed gamma- and beta-globin, they displayed a wide range of gamma-globin expression in relation to that of beta-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human gamma-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that gamma-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

Bibliography

Zitnik, G., Hines, P., Stamatoyannopoulos, G., & Papayannopoulou, T. (1991). Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes. Proceedings of the National Academy of Sciences, 88(6), 2530â2534.

Authors 4

G Zitnik (first)
P Hines (additional)
G Stamatoyannopoulos (additional)
T Papayannopoulou (additional)

References 0 Referenced 11

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:56 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:14 p.m.)
Indexed	1 year, 11 months ago (Sept. 22, 2023, 1:22 p.m.)
Issued	34 years, 5 months ago (March 15, 1991)
Published	34 years, 5 months ago (March 15, 1991)
Published Online	34 years, 5 months ago (March 15, 1991)
Published Print	34 years, 5 months ago (March 15, 1991)

Funders 0

None

BibTeX

@article{Zitnik_1991, title={Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.6.2530}, DOI={10.1073/pnas.88.6.2530}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zitnik, G and Hines, P and Stamatoyannopoulos, G and Papayannopoulou, T}, year={1991}, month=mar, pages={2530–2534} }

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