DOI: 10.1073/pnas.88.4.1247. Transposon-facilitated DNA sequencing.

10.1073/pnas.88.4.1247

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We describe here a transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating. A miniplasmid was designed to select against transposon insertions into the vector. Sites of transposon insertion are mapped by the polymerase chain reaction with bacterial overnight cultures providing the templates. A small set of plasmids with transposons spaced several hundred base pairs apart can then be sequenced. Sequencing primers corresponding to the transposon ends allow sequencing in both directions. Thus, the entire sequence of both strands can be easily determined.

Bibliography

Strathmann, M., Hamilton, B. A., Mayeda, C. A., Simon, M. I., Meyerowitz, E. M., & Palazzolo, M. J. (1991). Transposon-facilitated DNA sequencing. Proceedings of the National Academy of Sciences, 88(4), 1247â1250.

Authors 6

M Strathmann (first)
B A Hamilton (additional)
C A Mayeda (additional)
M I Simon (additional)
E M Meyerowitz (additional)
M J Palazzolo (additional)

References 0 Referenced 128

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:53 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:18 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 5, 2025, 8:19 a.m.)
Issued	34 years, 6 months ago (Feb. 15, 1991)
Published	34 years, 6 months ago (Feb. 15, 1991)
Published Online	34 years, 6 months ago (Feb. 15, 1991)
Published Print	34 years, 6 months ago (Feb. 15, 1991)

Funders 0

None

BibTeX

@article{Strathmann_1991, title={Transposon-facilitated DNA sequencing.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.4.1247}, DOI={10.1073/pnas.88.4.1247}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Strathmann, M and Hamilton, B A and Mayeda, C A and Simon, M I and Meyerowitz, E M and Palazzolo, M J}, year={1991}, month=feb, pages={1247–1250} }

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