DOI: 10.1073/pnas.88.22.10163. Efficient trans cleavage and a common structural motif for the ribozymes of the human hepatitis delta agent.

Efficient trans cleavage and a common structural motif for the ribozymes of the human hepatitis delta agent.

10.1073/pnas.88.22.10163

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cis-active ribozymes are potential therapeutic agents; however, to be used in this capacity, they must first be converted to trans-active ribozymes, a process facilitated by analysis of their structures. We present evidence that the genomic and antigenomic ribozymes of the human delta hepatitis agent share a structural ("axehead") motif that has conserved sequence elements and a stable hairpin. Guided by the features of the axehead, we divided each of the delta ribozymes into two subdomains, which we synthesized as separate RNA transcripts to give an enzyme and substrate for each ribozyme. Incubation of a substrate subdomain with its matching enzyme resulted in efficient and accurate trans cleavage. This work forms the basis for kinetic studies and for adapting the delta ribozymes for cleavage of selected target RNAs.

Bibliography

Branch, A. D., & Robertson, H. D. (1991). Efficient trans cleavage and a common structural motif for the ribozymes of the human hepatitis delta agent. Proceedings of the National Academy of Sciences, 88(22), 10163â10167.

Authors 2

A D Branch (first)
H D Robertson (additional)

References 0 Referenced 65

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:50 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:19 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:42 a.m.)
Issued	33 years, 9 months ago (Nov. 15, 1991)
Published	33 years, 9 months ago (Nov. 15, 1991)
Published Online	33 years, 9 months ago (Nov. 15, 1991)
Published Print	33 years, 9 months ago (Nov. 15, 1991)

Funders 0

None

BibTeX

@article{Branch_1991, title={Efficient trans cleavage and a common structural motif for the ribozymes of the human hepatitis delta agent.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.22.10163}, DOI={10.1073/pnas.88.22.10163}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Branch, A D and Robertson, H D}, year={1991}, month=nov, pages={10163–10167} }

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