DOI: 10.1073/pnas.88.21.9483. Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.

Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.

10.1073/pnas.88.21.9483

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The structure of the unligated human T-cell recombinant cyclophilin has been determined at 3 A resolution by multipole isomorphous replacement methods and refined at 2.5 A resolution to an R factor of 0.209. The root-mean-square errors of the bond lengths and bond angles are 0.013 A and 2.8 degrees from ideal geometry, respectively. The overall structure is a beta-barrel, consisting of eight antiparallel beta-strands wrapping around the barrel surface and two alpha-helices sitting on the top and the bottom closing the barrel. Inside the barrel, seven aromatic and other hydrophobic residues form a compact hydrophobic core. A loop of Lys-118 to His-126 and four beta-strands (B3-B6) constitute a pocket we speculate to be the binding site of cyclosporin A.

Bibliography

Ke, H. M., Zydowsky, L. D., Liu, J., & Walsh, C. T. (1991). Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution. Proceedings of the National Academy of Sciences, 88(21), 9483â9487.

Authors 4

H M Ke (first)
L D Zydowsky (additional)
J Liu (additional)
C T Walsh (additional)

References 0 Referenced 137

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:47 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:19 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 5, 2025, 9:08 a.m.)
Issued	33 years, 9 months ago (Nov. 1, 1991)
Published	33 years, 9 months ago (Nov. 1, 1991)
Published Online	33 years, 9 months ago (Nov. 1, 1991)
Published Print	33 years, 9 months ago (Nov. 1, 1991)

Funders 0

None

BibTeX

@article{Ke_1991, title={Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.21.9483}, DOI={10.1073/pnas.88.21.9483}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ke, H M and Zydowsky, L D and Liu, J and Walsh, C T}, year={1991}, month=nov, pages={9483–9487} }

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