DOI: 10.1073/pnas.88.16.7391. Localization of pre-messenger RNA at discrete nuclear sites.

Localization of pre-messenger RNA at discrete nuclear sites.

10.1073/pnas.88.16.7391

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have studied the nuclear localization of rhodamine-labeled pre-mRNA after microinjection into nuclei of cultured rat kidney epithelial cells. Intranuclear localization of the injected RNA was followed in the living cells by fluorescence microscopy and digital image processing. Injected human beta-globin pre-mRNA became localized in 30-60 discrete nuclear sites that were coincident with loci defined by monoclonal antibodies against small nuclear ribonucleoproteins (Sm) or another spliceosome component (SC-35) in parallel immunocytochemical studies on the same nuclei. Similar patterns of nuclear localization were observed with a rat proenkephalin pre-mRNA. Nuclear microinjection of an intronlacking beta-globin RNA, a splicing-defective beta-globin mutant pre-mRNA, or an antisense beta-globin pre-mRNA did not result in localization at discrete sites. These results indicate that pre-mRNA binds preferentially to nuclear Sm and SC-35 antibody-reactive sites in vivo and that the binding requires intron sequences.

Bibliography

Wang, J., Cao, L. G., Wang, Y. L., & Pederson, T. (1991). Localization of pre-messenger RNA at discrete nuclear sites. Proceedings of the National Academy of Sciences, 88(16), 7391â7395.

Authors 4

J Wang (first)
L G Cao (additional)
Y L Wang (additional)
T Pederson (additional)

References 0 Referenced 84

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:04 p.m.)
Indexed	3 months, 1 week ago (May 14, 2025, 2:43 p.m.)
Issued	34 years ago (Aug. 15, 1991)
Published	34 years ago (Aug. 15, 1991)
Published Online	34 years ago (Aug. 15, 1991)
Published Print	34 years ago (Aug. 15, 1991)

Funders 0

None

BibTeX

@article{Wang_1991, title={Localization of pre-messenger RNA at discrete nuclear sites.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.16.7391}, DOI={10.1073/pnas.88.16.7391}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, J and Cao, L G and Wang, Y L and Pederson, T}, year={1991}, month=aug, pages={7391–7395} }

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