DOI: 10.1073/pnas.88.10.4109. Heat shock-regulated transcription in vitro from a reconstituted chromatin template.

Heat shock-regulated transcription in vitro from a reconstituted chromatin template.

10.1073/pnas.88.10.4109

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

To investigate the mechanisms of transcriptional regulation of Drosophila heat shock genes we studied the activity of a heat shock promoter in vitro after reconstitution into chromatin. Increasing the duration of nucleosome assembly progressively inactivated a plasmid template when it was transcribed with extracts of either unshocked or heat-shocked Drosophila embryos, despite induction of the transcriptional activator heat shock factor. Addition of the general transcription factor IID (TFIID) before nucleosome assembly did not significantly relieve nucleosomal inhibition, but TFIID potentiated the promoter to be responsive to activation by heat shock factor in the heat shock transcription extract. The potentiation by TFIID could be related to the nucleosome-free, hypersensitive state of heat shock promoters previously observed in vivo before heat shock induction and may be necessitated by the need to expedite activation of heat shock genes in response to environmental stress.

Bibliography

Becker, P. B., Rabindran, S. K., & Wu, C. (1991). Heat shock-regulated transcription in vitro from a reconstituted chromatin template. Proceedings of the National Academy of Sciences, 88(10), 4109â4113.

Authors 3

P B Becker (first)
S K Rabindran (additional)
C Wu (additional)

References 0 Referenced 51

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:20 p.m.)
Indexed	1 year, 2 months ago (June 21, 2024, 6:08 a.m.)
Issued	34 years, 3 months ago (May 15, 1991)
Published	34 years, 3 months ago (May 15, 1991)
Published Online	34 years, 3 months ago (May 15, 1991)
Published Print	34 years, 3 months ago (May 15, 1991)

Funders 0

None

BibTeX

@article{Becker_1991, title={Heat shock-regulated transcription in vitro from a reconstituted chromatin template.}, volume={88}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.88.10.4109}, DOI={10.1073/pnas.88.10.4109}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Becker, P B and Rabindran, S K and Wu, C}, year={1991}, month=may, pages={4109–4113} }

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