DOI: 10.1073/pnas.87.8.3082. A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

10.1073/pnas.87.8.3082

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

Bibliography

Garcia-Blanco, M. A., Anderson, G. J., Beggs, J., & Sharp, P. A. (1990). A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein. Proceedings of the National Academy of Sciences, 87(8), 3082â3086.

Authors 4

M A Garcia-Blanco (first)
G J Anderson (additional)
J Beggs (additional)
P A Sharp (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:04 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:31 p.m.)
Issued	35 years, 5 months ago (April 1, 1990)
Published	35 years, 5 months ago (April 1, 1990)
Published Online	35 years, 5 months ago (April 1, 1990)
Published Print	35 years, 5 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Garcia_Blanco_1990, title={A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.8.3082}, DOI={10.1073/pnas.87.8.3082}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Garcia-Blanco, M A and Anderson, G J and Beggs, J and Sharp, P A}, year={1990}, month=apr, pages={3082–3086} }

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