DOI: 10.1073/pnas.87.4.1273. Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity.

Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity.

10.1073/pnas.87.4.1273

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have examined the role of Thr-286 autophosphorylation in the autoregulation of Ca2+/calmodulin-dependent protein kinase II. Using site-directed mutagenesis, we have substituted alanine or serine for Thr-286, or isoleucine for Arg-283, in the 50-kDa subunit of the kinase and expressed each protein in bacteria. Activation and autophosphorylation of all four enzymes were stringently dependent on Ca2+/calmodulin, indicating that neither Arg-283 nor Thr-286 is an absolute requirement for the pseudosubstrate inhibition of the enzyme. Autophosphorylation of the Ile-283 or Ala-286 enzyme generated little, if any, Ca2+/calmodulin-independent kinase activity, unlike the parent (Thr-286) or Ser-286 enzyme. The enzymes expressed in bacteria are predominantly monomeric, indicating that the generation of Ca2+/calmodulin-independent activity does not require the cooperative interactions of subunits normally present in the brain holoenzyme.

Bibliography

Waxham, M. N., Aronowski, J., Westgate, S. A., & Kelly, P. T. (1990). Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity. Proceedings of the National Academy of Sciences, 87(4), 1273â1277.

Authors 4

M N Waxham (first)
J Aronowski (additional)
S A Westgate (additional)
P T Kelly (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:33 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:04 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 1 p.m.)
Issued	35 years, 6 months ago (Feb. 1, 1990)
Published	35 years, 6 months ago (Feb. 1, 1990)
Published Online	35 years, 6 months ago (Feb. 1, 1990)
Published Print	35 years, 6 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Waxham_1990, title={Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.4.1273}, DOI={10.1073/pnas.87.4.1273}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Waxham, M N and Aronowski, J and Westgate, S A and Kelly, P T}, year={1990}, month=feb, pages={1273–1277} }

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