DOI: 10.1073/pnas.87.23.9158. Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.

Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.

10.1073/pnas.87.23.9158

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have identified and partially characterized another human general transcription factor, TFIIG. Using a reconstituted in vitro system comprised of purified RNA polymerase II, TFIIB, TFIID, TFIIE, and TFIIF, we found that TFIIG was essential for specific initiation from all class II genes tested. In this system TFIIA could partially replace TFIIG; however, even at saturating concentrations of TFIIA, addition of TFIIG further stimulated transcription. Since the chromatographic properties of TFIIG differed significantly from those of TFIIA, we concluded that TFIIA and TFIIG are distinct but functionally related transcription factors. Heparin challenge assays showed that TFIIG is required for the assembly of a functional preinitiation complex. However, it must act after template commitment by TFIID, since this step did not require, and was unaffected by, either TFIIG or TFIIA.

Bibliography

Sumimoto, H., Ohkuma, Y., Yamamoto, T., Horikoshi, M., & Roeder, R. G. (1990). Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG. Proceedings of the National Academy of Sciences, 87(23), 9158â9162.

Authors 5

H Sumimoto (first)
Y Ohkuma (additional)
T Yamamoto (additional)
M Horikoshi (additional)
R G Roeder (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:30 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:10 p.m.)
Indexed	1 year, 2 months ago (May 31, 2024, 7:37 p.m.)
Issued	34 years, 8 months ago (Dec. 1, 1990)
Published	34 years, 8 months ago (Dec. 1, 1990)
Published Online	34 years, 8 months ago (Dec. 1, 1990)
Published Print	34 years, 8 months ago (Dec. 1, 1990)

Funders 0

None

BibTeX

@article{Sumimoto_1990, title={Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.23.9158}, DOI={10.1073/pnas.87.23.9158}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sumimoto, H and Ohkuma, Y and Yamamoto, T and Horikoshi, M and Roeder, R G}, year={1990}, month=dec, pages={9158–9162} }

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