DOI: 10.1073/pnas.87.20.7912. Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins.

Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins.

10.1073/pnas.87.20.7912

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Two proteins, termed epithelin 1 and epithelin 2, that inhibit the growth of A431 cells, derived from a human epidermal carcinoma of the vulva, have been purified from rat kidney. Epithelin 1 stimulates the proliferation of murine keratinocytes, whereas epithelin 2 inhibits the epithelin 1-elicited growth of these cells. Thus epithelin 1 and 2 behave as agonist and antagonist, respectively, for normal epithelial cells. Epithelins are low molecular mass (approximately 6 kDa), acid- and heat-stable, single-chain proteins containing approximately 20% cysteine. Some of these cysteines form disulfide linkage(s) that are essential for biological activity. The amino-terminal amino acid sequences of epithelin 1 and epithelin 2 have been determined. The two proteins showed no substantial sequence homology with other proteins. However, a significant homology was seen between the amino-terminal sequences of epithelin 1 and epithelin 2. Epithelins 1 and 2, therefore, appear to represent members of a distinct family of growth regulators.

Bibliography

Shoyab, M., McDonald, V. L., Byles, C., Todaro, G. J., & Plowman, G. D. (1990). Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins. Proceedings of the National Academy of Sciences, 87(20), 7912â7916.

Authors 5

M Shoyab (first)
V L McDonald (additional)
C Byles (additional)
G J Todaro (additional)
G D Plowman (additional)

References 0 Referenced 150

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:12 p.m.)
Indexed	5 days, 22 hours ago (Aug. 28, 2025, 8:34 a.m.)
Issued	34 years, 11 months ago (Oct. 1, 1990)
Published	34 years, 11 months ago (Oct. 1, 1990)
Published Online	34 years, 11 months ago (Oct. 1, 1990)
Published Print	34 years, 11 months ago (Oct. 1, 1990)

Funders 0

None

BibTeX

@article{Shoyab_1990, title={Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.20.7912}, DOI={10.1073/pnas.87.20.7912}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shoyab, M and McDonald, V L and Byles, C and Todaro, G J and Plowman, G D}, year={1990}, month=oct, pages={7912–7916} }

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