DOI: 10.1073/pnas.87.19.7603. Direct detection of linker DNA bending in defined-length oligomers of chromatin.

Direct detection of linker DNA bending in defined-length oligomers of chromatin.

10.1073/pnas.87.19.7603

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Linker DNA, which connects between nucleosomes in chromatin, is short and, therefore, may be essentially straight and inflexible. We have carried out hydrodynamic and electron microscopic studies of dinucleosomes--fragments of chromatin containing just two nucleosomes--to test the ability of linker DNA to bend. We find that ionic conditions that stabilize the folding of long chromatin cause linker DNA in dinucleosomes to bend, bringing the two nucleosomes into contact. The results uphold a key prediction of the solenoid model of chromosome folding and suggest a mechanism by which proteins that are separated along the DNA can interact by direct contact.

Bibliography

Yao, J., Lowary, P. T., & Widom, J. (1990). Direct detection of linker DNA bending in defined-length oligomers of chromatin. Proceedings of the National Academy of Sciences, 87(19), 7603â7607.

Authors 3

J Yao (first)
P T Lowary (additional)
J Widom (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:27 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:24 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	34 years, 10 months ago (Oct. 1, 1990)
Published	34 years, 10 months ago (Oct. 1, 1990)
Published Online	34 years, 10 months ago (Oct. 1, 1990)
Published Print	34 years, 10 months ago (Oct. 1, 1990)

Funders 0

None

BibTeX

@article{Yao_1990, title={Direct detection of linker DNA bending in defined-length oligomers of chromatin.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.19.7603}, DOI={10.1073/pnas.87.19.7603}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yao, J and Lowary, P T and Widom, J}, year={1990}, month=oct, pages={7603–7607} }

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