DOI: 10.1073/pnas.87.11.4164. Integration of human immunodeficiency virus type 1 DNA in vitro.

Integration of human immunodeficiency virus type 1 DNA in vitro.

10.1073/pnas.87.11.4164

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.

Bibliography

Farnet, C. M., & Haseltine, W. A. (1990). Integration of human immunodeficiency virus type 1 DNA in vitro. Proceedings of the National Academy of Sciences, 87(11), 4164â4168.

Authors 2

C M Farnet (first)
W A Haseltine (additional)

References 0 Referenced 204

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:20 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:51 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 9:31 a.m.)
Issued	35 years, 3 months ago (June 1, 1990)
Published	35 years, 3 months ago (June 1, 1990)
Published Online	35 years, 3 months ago (June 1, 1990)
Published Print	35 years, 3 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{Farnet_1990, title={Integration of human immunodeficiency virus type 1 DNA in vitro.}, volume={87}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.87.11.4164}, DOI={10.1073/pnas.87.11.4164}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Farnet, C M and Haseltine, W A}, year={1990}, month=jun, pages={4164–4168} }

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