DOI: 10.1073/pnas.86.22.8635. Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.

Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.

10.1073/pnas.86.22.8635

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The structure of isocitrate dehydrogenase [threo-DS-isocitrate: NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42] from Escherichia coli has been solved and refined at 2.5 A resolution and is topologically different from that of any other dehydrogenase. This enzyme, a dimer of identical 416-residue subunits, is inactivated by phosphorylation at Ser-113, which lies at the edge of an interdomain pocket that also contains many residues conserved between isocitrate dehydrogenase and isopropylmalate dehydrogenase. Isocitrate dehydrogenase contains an unusual clasp-like domain in which both polypeptide chains in the dimer interlock. Based on the structure of isocitrate dehydrogenase and conservation with isopropylmalate dehydrogenase, we suggest that the active site lies in an interdomain pocket close to the phosphorylation site.

Bibliography

Hurley, J. H., Thorsness, P. E., Ramalingam, V., Helmers, N. H., Koshland, D. E., & Stroud, R. M. (1989). Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proceedings of the National Academy of Sciences, 86(22), 8635â8639.

Authors 6

J H Hurley (first)
P E Thorsness (additional)
V Ramalingam (additional)
N H Helmers (additional)
D E Koshland (additional)
R M Stroud (additional)

References 0 Referenced 157

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 7:07 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:51 p.m.)
Indexed	1 day, 17 hours ago (Sept. 4, 2025, 9:50 a.m.)
Issued	35 years, 10 months ago (Nov. 1, 1989)
Published	35 years, 10 months ago (Nov. 1, 1989)
Published Online	35 years, 10 months ago (Nov. 1, 1989)
Published Print	35 years, 10 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Hurley_1989, title={Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.}, volume={86}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.86.22.8635}, DOI={10.1073/pnas.86.22.8635}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hurley, J H and Thorsness, P E and Ramalingam, V and Helmers, N H and Koshland, D E and Stroud, R M}, year={1989}, month=nov, pages={8635–8639} }

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