DOI: 10.1073/pnas.86.18.6858. In vitro attachment of glycosyl-inositolphospholipid anchor structures to mouse Thy-1 antigen and human decay-accelerating factor.

In vitro attachment of glycosyl-inositolphospholipid anchor structures to mouse Thy-1 antigen and human decay-accelerating factor.

10.1073/pnas.86.18.6858

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Glycosyl-inositolphospholipid (GPL) anchoring structures are incorporated into GPL-anchored proteins immediately posttranslationally in the rough endoplasmic reticulum, but the biochemical and cellular constituents involved in this "glypiation" process are unknown. To establish whether glypiation could be achieved in vitro, mRNAs generated by transcription of cDNAs encoding two GPL-anchored proteins, murine Thy-1 antigen and human decay-accelerating factor (DAF), and a conventionally anchored control protein, polymeric-immunoglobulin receptor (IgR), were translated in a rabbit reticulocyte lysate. Upon addition of dog pancreatic rough microsomes, nascent polypeptides generated from the three mRNAs translocated into vesicles. Dispersal of the vesicles with Triton X-114 detergent and incubation of the hydrophobic phase with phosphatidylinositol-specific phospholipases C and D, enzymes specific for GPL-anchor structures, released Thy-1 and DAF but not IgR protein into the aqueous phase. The selective incorporation of phospholipase-sensitive anchoring moieties into Thy-1 and DAF but not IgR translation products during in vitro translocation indicates that rough microsomes are able to support and regulate glypiation.

Bibliography

Fasel, N., Rousseaux, M., Schaerer, E., Medof, M. E., Tykocinski, M. L., & Bron, C. (1989). In vitro attachment of glycosyl-inositolphospholipid anchor structures to mouse Thy-1 antigen and human decay-accelerating factor. Proceedings of the National Academy of Sciences, 86(18), 6858â6862.

Authors 6

N Fasel (first)
M Rousseaux (additional)
E Schaerer (additional)
M E Medof (additional)
M L Tykocinski (additional)
C Bron (additional)

References 0 Referenced 17

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 7:03 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:43 p.m.)
Indexed	1 year, 2 months ago (June 11, 2024, 4:31 a.m.)
Issued	35 years, 11 months ago (Sept. 1, 1989)
Published	35 years, 11 months ago (Sept. 1, 1989)
Published Online	35 years, 11 months ago (Sept. 1, 1989)
Published Print	35 years, 11 months ago (Sept. 1, 1989)

Funders 0

None

BibTeX

@article{Fasel_1989, title={In vitro attachment of glycosyl-inositolphospholipid anchor structures to mouse Thy-1 antigen and human decay-accelerating factor.}, volume={86}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.86.18.6858}, DOI={10.1073/pnas.86.18.6858}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fasel, N and Rousseaux, M and Schaerer, E and Medof, M E and Tykocinski, M L and Bron, C}, year={1989}, month=sep, pages={6858–6862} }

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