DOI: 10.1073/pnas.86.12.4397. tRNA-tRNA interactions within cellular ribosomes.

tRNA-tRNA interactions within cellular ribosomes.

10.1073/pnas.86.12.4397

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We describe an assay that converts the effects of tRNA-tRNA contacts at two particular codons into a quantitative effect on beta-galactosidase level. The assay measures the separate and combined efficiency of suppression at adjacent nonsense codons in vivo using a set of specially created homologous messages. In a survey of distal anticodon arm substitutions, we find that particular mutant tRNAs occupying the P-site reduce the apparent efficiency of the suppressor tRNA reading the A-site codon by factors of 2-170. By using measured tRNA-tRNA distances and the crystallographic tRNA structure, we propose a model of the tRNA-tRNA-mRNA complex. In the model, the anticodon loops of the P-site and A-site tRNAs contact one another in a way that is consistent with our combined tRNA efficiency data. These results suggest that tRNA-tRNA interactions that modulate tRNA action are an inevitable feature of translation.

Bibliography

Smith, D., & Yarus, M. (1989). tRNA-tRNA interactions within cellular ribosomes. Proceedings of the National Academy of Sciences, 86(12), 4397â4401.

Authors 2

D Smith (first)
M Yarus (additional)

References 0 Referenced 76

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:56 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:01 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:43 a.m.)
Issued	36 years, 3 months ago (June 1, 1989)
Published	36 years, 3 months ago (June 1, 1989)
Published Online	36 years, 3 months ago (June 1, 1989)
Published Print	36 years, 3 months ago (June 1, 1989)

Funders 0

None

BibTeX

@article{Smith_1989, title={tRNA-tRNA interactions within cellular ribosomes.}, volume={86}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.86.12.4397}, DOI={10.1073/pnas.86.12.4397}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Smith, D and Yarus, M}, year={1989}, month=jun, pages={4397–4401} }

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