DOI: 10.1073/pnas.85.8.2608. Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts.

Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts.

10.1073/pnas.85.8.2608

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have used anti-phosphotyrosine antibodies to identify a large number of tyrosine phosphoproteins in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. Most of these proteins were found in the 100,000 X g supernatant when cells were separated into soluble and particulate fractions; however, one group of proteins, of 115-120 kDa, was found in the particulate fraction. The phosphotyrosine content of the 115- to 120-kDa proteins was greatly reduced in chicken embryo fibroblasts infected with mutants of RSV (NY314 and SD10) encoding nonmyristoylated forms of the viral src gene product that do not associate with cellular membranes. Since RSV mutants NY314 and SD10 do not transform cells, phosphorylation of this group of 115- to 120-kDa membrane proteins may be related to the process of transformation.

Bibliography

Linder, M. E., & Burr, J. G. (1988). Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts. Proceedings of the National Academy of Sciences, 85(8), 2608â2612.

Authors 2

M E Linder (first)
J G Burr (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:53 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:45 p.m.)
Indexed	1 year, 8 months ago (Dec. 23, 2023, 7:27 p.m.)
Issued	37 years, 4 months ago (April 1, 1988)
Published	37 years, 4 months ago (April 1, 1988)
Published Online	37 years, 4 months ago (April 1, 1988)
Published Print	37 years, 4 months ago (April 1, 1988)

Funders 0

None

BibTeX

@article{Linder_1988, title={Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.8.2608}, DOI={10.1073/pnas.85.8.2608}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Linder, M E and Burr, J G}, year={1988}, month=apr, pages={2608–2612} }

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