DOI: 10.1073/pnas.85.8.2558. Thymine dimers bend DNA.

10.1073/pnas.85.8.2558

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A 32-base-pair DNA fragment containing a thymine photodimer was constructed and ligated head-to-tail to obtain multimers of this sequence in which thymine dimers were in phase with the helix screw axis (approximately equal to 3 turns apart). The ligation products were analyzed by one- and two-dimensional gel electrophoresis and quantitative electron microscopy. These analyses show that the thymine photodimer introduces a bend of approximately equal to 30 degrees in DNA, which causes anomalously slow migration of DNA fragments in polyacrylamide gels and facilitates the formation of small covalent circles. Repair of thymine dimers by DNA photolyase abolishes the anomalous migration.

Bibliography

Husain, I., Griffith, J., & Sancar, A. (1988). Thymine dimers bend DNA. Proceedings of the National Academy of Sciences, 85(8), 2558â2562.

Authors 3

I Husain (first)
J Griffith (additional)
A Sancar (additional)

References 0 Referenced 115

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:53 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:47 p.m.)
Indexed	2 months ago (July 1, 2025, 6:30 a.m.)
Issued	37 years, 5 months ago (April 1, 1988)
Published	37 years, 5 months ago (April 1, 1988)
Published Online	37 years, 5 months ago (April 1, 1988)
Published Print	37 years, 5 months ago (April 1, 1988)

Funders 0

None

BibTeX

@article{Husain_1988, title={Thymine dimers bend DNA.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.8.2558}, DOI={10.1073/pnas.85.8.2558}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Husain, I and Griffith, J and Sancar, A}, year={1988}, month=apr, pages={2558–2562} }

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