DOI: 10.1073/pnas.85.5.1492. Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

10.1073/pnas.85.5.1492

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Genomic clones encoding the A chain of platelet-derived growth factor (PDGF) have been isolated. The gene contains seven exons spanning about 24 kilobases of DNA. The positions of intervening sequences closely match those of the related B-chain (c-sis) gene on chromosome 22. In situ hybridization was used to localize the PDGF A-chain gene to the distal portion of the short arm of chromosome 7 (7p21-p22). Within the (G + C)-rich 5' region, a single transcriptional start site was identified approximately equal to 36 base pairs downstream of a TATAA consensus promoter element. The three size classes of A-chain mRNA probably arise by selection of alternative poly(A) sites in exon 7, but only a single consensus AATAAA signal was identified in this region. Two functionally different A-chain precursors, which differ by the presence or absence of a basic C terminus, are generated as a result of alternative mRNA splicing events, which include or exclude exon 6. This and other structural features of the A-chain gene suggest that PDGF expression may be modulated at transcriptional and post-transcriptional levels.

Bibliography

Bonthron, D. T., Morton, C. C., Orkin, S. H., & Collins, T. (1988). Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing. Proceedings of the National Academy of Sciences, 85(5), 1492â1496.

Authors 4

D T Bonthron (first)
C C Morton (additional)
S H Orkin (additional)
T Collins (additional)

References 0 Referenced 113

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:51 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:41 p.m.)
Indexed	2 months, 3 weeks ago (June 11, 2025, 5:29 a.m.)
Issued	37 years, 6 months ago (March 1, 1988)
Published	37 years, 6 months ago (March 1, 1988)
Published Online	37 years, 6 months ago (March 1, 1988)
Published Print	37 years, 6 months ago (March 1, 1988)

Funders 0

None

BibTeX

@article{Bonthron_1988, title={Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.5.1492}, DOI={10.1073/pnas.85.5.1492}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bonthron, D T and Morton, C C and Orkin, S H and Collins, T}, year={1988}, month=mar, pages={1492–1496} }

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