DOI: 10.1073/pnas.85.4.1282. Laminin receptors for neurite formation.

Laminin receptors for neurite formation.

10.1073/pnas.85.4.1282

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Laminin, a basement membrane glycoprotein promotes both cell attachment and neurite outgrowth. Separate domains on laminin elicit these responses, suggesting that distinct receptors occur on the surface of cells. NG108-15 neuroblastoma-glioma cells rapidly extend long processes in the presence of laminin. We report here that 125I-labeled laminin specifically binds to these cells and to three membrane proteins of 67, 110, and 180 kDa. These proteins were isolated by affinity chromatography on laminin-Sepharose. The 67-kDa protein reacted with antibody to the previously characterized receptor for cell attachment to laminin. Antibodies to the 110-kDa and 180-kDa bands demonstrated that the 110-kDa protein was found in a variety of epithelial cell lines and in brain, whereas the 180-kDa protein was neural specific. Antibodies prepared against the 110-kDa and 180-kDa proteins inhibited neurite outgrowth induced by the neurite-promoting domain of laminin, whereas antibodies to the 67-kDa laminin receptor had no effect on neurite outgrowth. We conclude that neuronal cells have multiple cell-surface laminin receptors and that the 110-kDa and 180-kDa proteins are involved in neurite formation.

Bibliography

Kleinman, H. K., Ogle, R. C., Cannon, F. B., Little, C. D., Sweeney, T. M., & Luckenbill-Edds, L. (1988). Laminin receptors for neurite formation. Proceedings of the National Academy of Sciences, 85(4), 1282â1286.

Authors 6

H K Kleinman (first)
R C Ogle (additional)
F B Cannon (additional)
C D Little (additional)
T M Sweeney (additional)
L Luckenbill-Edds (additional)

References 0 Referenced 111

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:49 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:37 p.m.)
Indexed	1 month, 3 weeks ago (July 1, 2025, 9:31 a.m.)
Issued	37 years, 6 months ago (Feb. 1, 1988)
Published	37 years, 6 months ago (Feb. 1, 1988)
Published Online	37 years, 6 months ago (Feb. 1, 1988)
Published Print	37 years, 6 months ago (Feb. 1, 1988)

Funders 0

None

BibTeX

@article{Kleinman_1988, title={Laminin receptors for neurite formation.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.4.1282}, DOI={10.1073/pnas.85.4.1282}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kleinman, H K and Ogle, R C and Cannon, F B and Little, C D and Sweeney, T M and Luckenbill-Edds, L}, year={1988}, month=feb, pages={1282–1286} }

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