DOI: 10.1073/pnas.85.4.1028. Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.

Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.

10.1073/pnas.85.4.1028

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The human promyelocytic leukemia cell line HL-60 overexpresses the c-myc protooncogene. A calculated secondary structure for c-myc mRNA placed the initiation codon in a bulge of a weakly base-paired region. Treatment of HL-60 cells with 5' d(AACGTTGAGGGGCAT) 3', complementary to the initiation codon and the next four codons of c-myc mRNA, inhibited c-myc protein expression in a dose-dependent manner. However, treatment of HL-60 cells with 5' d(TTGGGATAACACTTA) 3', complementary to nucleotides 17-31 of vesicular stomatitis virus matrix protein mRNA, displayed no such effects. These results agree with analogous studies of normal human T lymphocytes [Heikkila, R., Schwab, G., Wickstrom, E., Loke, S. L., Pluznik, D. H., Watt, R. & Neckers, L. M. (1987) Nature (London) 328, 445-449], except that only one-third as much oligomer was needed for a comparable effect. Proliferation of HL-60 cells in culture was inhibited in a sequence-specific, dose-dependent manner by the c-myc-complementary oligomer, but neither the oligomer complementary to vesicular stomatitis virus matrix protein mRNA nor 5' d(CATTTCTTGCTCTCC) 3', complementary to nucleotides 5399-5413 of human immunodeficiency virus tat gene mRNA, inhibited proliferation. It thus appears that antisense oligodeoxynucleotides added to myc-transformed cells via culture medium are capable of eliciting sequence-specific, dose-dependent inhibition of c-myc protein expression and cell proliferation.

Bibliography

Wickstrom, E. L., Bacon, T. A., Gonzalez, A., Freeman, D. L., Lyman, G. H., & Wickstrom, E. (1988). Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA. Proceedings of the National Academy of Sciences, 85(4), 1028â1032.

Authors 6

E L Wickstrom (first)
T A Bacon (additional)
A Gonzalez (additional)
D L Freeman (additional)
G H Lyman (additional)
E Wickstrom (additional)

References 0 Referenced 224

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:49 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:38 p.m.)
Indexed	4 weeks ago (Aug. 2, 2025, 12:57 a.m.)
Issued	37 years, 6 months ago (Feb. 1, 1988)
Published	37 years, 6 months ago (Feb. 1, 1988)
Published Online	37 years, 6 months ago (Feb. 1, 1988)
Published Print	37 years, 6 months ago (Feb. 1, 1988)

Funders 0

None

BibTeX

@article{Wickstrom_1988, title={Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.4.1028}, DOI={10.1073/pnas.85.4.1028}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wickstrom, E L and Bacon, T A and Gonzalez, A and Freeman, D L and Lyman, G H and Wickstrom, E}, year={1988}, month=feb, pages={1028–1032} }

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